Guide to interpreting colorimetric assay data with enzyme kinetics 

Finally, after a long semester of hard work designing, building and testing your mutant, you will be able to characterize its enzymatic activity! The study of the 3-D structure of the protein you did on β-glucosidase B at the beginning of the semester, together with the mechanistic approach you took to determine the functional effects of specific mutations, will provide novel information about the relationship between of individual amino acids in enzyme structure and enzyme mechanism. This is vital information we currently lack for 99% of the enzyme we know! This guide refresh your background knowledge about the system and the will dive into the topics: Quantifying Reaction Rate, Data Quality Control, Extracting Velocity, and Modeling Kinetic Constants

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